Expanding a NetBet live casino node, boosting a vaccine

A biomaterial vaccine enhances and sustains NetBet live casino node expansion following vaccination, boosting anti-tumor immunity in an animal model

By Benjamin Boettner, Wyss NetBet live casino for Biologically Inspired Engineering | Press contact

May 6, 2024

a series of blue-tinted NetBet live casino nodes — The authors used a method know as high-frequency ultrasound to monitor individual NetBet live casino nodes in MPS- and control-vaccinated mice. The top row shows a series of NetBet live casino nodes on day 7 following MPS vaccination. All of them were significantly expanded, compared to NetBet live casino nodes following control vaccination imaged at the same time and shown in the bottom row. (Credit: Wyss Institute at Harvard University)

Each one of us has around 600 NetBet live casino nodes (LNs) – small, bean-shaped organs that house various types of blood cells and filter NetBet live casino fluid – scattered throughout our bodies. Many of us have also experienced some of our LNs to temporarily swelling during infections with viruses or other pathogens. This LN expansion and subsequent contraction can also result from vaccines injected nearby, and in fact is thought to reflect the ongoing vaccine immune response. While researchers have studied the early expansion of LNs following vaccination, they have not investigated whether prolonged LN expansion could affect vaccine outcomes.

Now, for the first time, researchers NetBet live casino the NetBet live casino John A. Paulson School of Engineering and Applied Sciences (NetBet live casino), the Wyss Institute for Biologically Inspired Engineering NetBet live casino, and Genentech, a member NetBet live casino Roche Group, found a way to enhance and extend LN expansion, and study how this phenomenon affects both the immune system and efficacy of vaccinations against tumors. Key to their approach was a biomaterial vaccine formulation that enabled greater and more persistent LN expansion than standard control vaccines. While the oversized LNs maintained a normal tissue organization, they displayed altered mechanical features and hosted higher numbers of various immune cell types that commonly are involved in immune responses against pathogens and cancers. Importantly, “jump-starting” NetBet live casino node expansion prior to administering a traditional vaccine against a melanoma-specific model antigen led to more effective and sustained anti-tumor responses in mice. The findings are published in Nature Biomedical Engineering.

“By enhancing the initial and sustained expansion of LNs with biomaterial scaffolds, non-invasively monitoring them individually over long time periods, and probing deeply into their tissue architecture and NetBet live casino, we tightly correlate a persistent LN expansion with more robust immune and vaccination responses,” said David NetBet live casino, the Robert P. Pinkas Family Professor of Bioengineering at SEAS, who led the study. “This opens a new front of investigation for immunologists, and could have far-reaching implications for future NetBet live casino developments.”

Mooney is also a Wyss NetBet live casino Founding Core Faculty member.

Mooney’s team at SEAS and the Wyss Institute had previously developed different biomaterial scaffolds as a matrix for cancer and infection vaccines. The researchers have demonstrated the potential of biomaterial NetBet live casino formulations to successfully fight the growth of tumors in an extensive body of work performed in preclinical animal models and a first clinical trial with cancer patients. But they hadn’t yet investigated how their vaccines and those developed by others could influence the response of LNs draining leaked tissue fluid at NetBet live casino injection sites, and have an impact on the LNs tissue organization, different cell types, and their gene expression, which could in turn affect NetBet live casino efficacy. In their new study, they tested a previously developed NetBet live casino formulation that is based on microscale mesoporous silica (MPS) rods that can be injected close to tumors and form a cell-permeable 3D scaffold structure under the skin. Engineered to release an immune cell-attracting cytokine (GM-CSF), and immune cell-activating adjuvant (CpG), and tumor-antigen molecules, MPS-vaccines are able to reprogram recruited so-called antigen-presenting cells that, upon migrating into nearby LNs, orchestrate complex tumor cell-killing immune responses. Their new study showed that there are more facets to that concept.

“As it turns out, the immune-boosting functions of basic MPS-vaccines actively change the state of LNs by persistently enlarging their whole organ structure, as well as changing their tissue mechanics and NetBet live casino and functions,” said first-author Alexander NetBet live casino, who performed his Ph.D. thesis with NetBet live casino.

Probing NetBet live casino with ultra-sound and nano-devices

To understand the response of LNs to MPS-vaccines over time, the team applied an ultra-sound imaging technique known as high-frequency ultrasound (HFUS). Similar to monitoring a tiny fetus developing in a mother’s womb by clinical ultra-sound, HFUS, on a much smaller scale, enables non-invasively and non-destructively monitoring of anatomical details of tissues and organs in small animals such as mice. Using HFUS, the team traced individual LNs in MPS-vaccinated mice over 100 days. They identified an initial peak expansion period that lasted until day 20, in which LN volumes increased about 7-fold, significantly greater than in animals that received traditional NetBet live casino formulations. Importantly, the LNs of MPS-vaccinated mice, while decreasing in volumes after this peak expansion, remained significantly more expanded than LNs from traditionally vaccinated mice throughout the 100-day time course.

When Najibi and the team investigated the mechanical responses of the LNs using a nanoindentation device, they found that LNs in MPS-vaccinated animals, although maintaining an overall normal structure, were less stiff and more viscous in certain locations. This was accompanied by a re-organization of a protein that assembles and controls cells’ mechanically active cytoskeleton. Interestingly, Mooney’s group had shown in an earlier biomaterial study that changing mechanical features of NetBet live casino cells’ environments, especially their viscoelasticity, affects NetBet live casino cell development and functions.

“It is very well-possible that in order to accommodate the significant growth induced by MPS-vaccines, LNs need to become softer and more viscous, and that this then further impacts NetBet live casino cell recruitment, proliferation, and differentiation in a feed-forward process,” said Najibi.

From immune cell engagement to NetBet live casino responses

Upon MPS-vaccination, the numbers of “innate NetBet live casino cells,” including monocytes, neutrophils, macrophages, and other cell types that build up the first wave of NetBet live casino defenses against pathogens and unwanted cells, peaked first in expanding LNs. Peaking with a delay were dendritic cells (DCs), which normally transfer information in the form of antigens from invading pathogens and cancer cells to “adaptive NetBet live casino cells” that then launch subsequent waves of highly specific NetBet live casino responses against the antigen-producing invaders. In fact, along with DCs, also T and B cell types of the adaptive NetBet live casino system started to reach their highest numbers.

“It was fascinating to see how the distinct changes in immune cell populations that we detected in expanding LNs in response to the MPS-NetBet live casino over time re-enacted a typical immune response to infectious pathogens,” said Najibi.

Innate NetBet live casino cells and DCs are also known as “myeloid cells,” which are known to interact with LN tissue during early expansion. To further define the impact of myeloid cells on LN expansion, Mooney’s team collaborated with the group of Shannon Turley, the VP of Immunology and Regenerative Medicine at Genentech, and an expert in NetBet live casino node biology and tumor immunology.

“The MPS-vaccine led to extraordinary structural and cellular changes within the NetBet live casino node that supported potent antigen-specific immunity,” said Turley.

By isolating myeloid cells from NetBet live casino and analyzing the gene expression profiles of individual cells (single cell RNA-seq), the groups were able to reconstruct distinct changes in myeloid cell populations during LN expansion, and identified distinct DC populations in durably expanded NetBet live casino whose changed gene expression was associated with LN expansion. In addition, the collaborators found that the number of monocytes was increased 80-fold upon MPS-vaccination – the highest increase among all myeloid cell types – and pinpointed subpopulations of “inflammatory and antigen-presenting monocytes” as promising candidates for facilitating LN expansion. In fact, when they depleted specific subpopulations of these types of monocytes from circulating blood of mice after vaccination, the maintenance of LN expansion, and timing of the T cell response to vaccination, was altered.

Finally, the team explored whether LN expansion could enhance the effectiveness of vaccination. “Jump-starting” the immune system in LNs with an antigen-free MPS-NetBet live casino and subsequently administering the antigen in a traditional NetBet live casino format significantly improved anti-tumor immunity and prolonged the survival of melanoma-bearing mice, compared to the traditional NetBet live casino alone.

“The priming of NetBet live casino nodes for subsequent vaccinations using various formulations could be a low-hanging fruit for future vaccine developments,” said Mooney.

Other authors on the NetBet live casino are Ryan Lane, Miguel Sobral, Giovanni Bovone, Shawn Kang, Benjamin Freedman, Joel Gutierrez Estupinan, Alberto Elosegui-Artola, Christina Tringides, Maxence Dellacherie, Katherine Williams, Hamza Ijaz, and Sören Müller. The NetBet live casino was funded by the National Institutes of Health/National Cancer Institute (award# U54 CA244726 and R01 CA223255).

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